Chimerism und MRD Diagnostic

Molecular biologic analyses have become an integral part of diagnostic and follow-up investigation of live threatening malignant and non-malignant diseases, thus completing cytogenetic and immunological investigations.

Our laboratory supports the evaluation of treatment follow-up mainly in patients after allogeneic blood stem cell transplantation. After enrichment of special cellular fractions and cell subpopulations we apply qualitative and quantitative PCR procedures, fragment analysis and nucleotide sequencing.

The focus of our laboratory is the analysis of chimerism after allogeneic stem cell transplantation and the analysis of minimal residual disease (MRD) in patients with acute lymphoblastic leukemia. Both procedures are certified according to DIN EN ISO 15189 (Certificate). The laboratory is certified according to DIN EN ISO 9001:2008, it is member of the Euro-chimerism consortium and member of ESLOH EuroMRD-ALL.

Further procedures

  • Identity analysis (for example of cultured cells, cell lines or tumour samples)
     
  • Determination of feto-maternal microchimerism by means of Real-Time PCR
     
  • Vß-spectratyping to control immune reconstitution
     
  • Quantitative analysis of WT1 expression
     
  • Sequencing analysis